MensSana: Design of a mental well-being self-report interface for shop floor workers

A ascensão da Indústria 4.0 trouxe consigo novas tecnologias e oportunidades que estão a mudar a natureza do trabalho, especialmente em ambientes de chão de fábrica. No entanto, essas mudanças também trouxeram novos desafios para os trabalhadores, incluindo desafios na sua saúde mental. Estes trabalhadores, em particular, enfrentam no seu trabalho estressores físicos e mentais que podem afetar seu bem-estar geral, apesar dos esforços da Indústria 4.0. O conceito de Operador 4.0 na Indústria 4.0 introduz muitos operadores, como o Operador Saudável, que enfatiza a centralidade no ser humano e visa melhorar a eficiência e o bem-estar do trabalhador por meio de tecnologias avançadas e análise de dados. Esta tese propõe o desenvolvimento de uma ferramenta protótipo, co-criada e validada no contexto da Indústria 4.0 para medir métricas do trabalhador e do local de trabalho, criando uma imagem holística do trabalhador, sua competência e bem-estar, alinhado ao conceito de um trabalhador "mais saudável" de Romero et al. Essas informações são devolvidas ao trabalhador e apresentadas de maneira legível e compreensível para identificar tendências e informar decisões futuras relacionadas ao trabalho e bem-estar.The rise of Industry 4.0 has brought about new technologies and opportunities that are changing the nature of work, particularly in factory floor settings. However, these changes have also brought about new challenges for workers, including mental health issues. Shop floor workers, in particular, face physical and mental stressors in their work that can impact their overall well-being, despite Industry 4.0 efforts. The Operator 4.0 concept in Industry 4.0 introduces a lot of operators like the Healthy Operator that emphasises human-centricity and aims to improve worker efficiency and well-being through advanced technologies and data analytics. This thesis proposes the development of a prototype tool co-created and validated in the context of Industry 4.0 to measure metrics from the worker and the workplace, creating a holistic picture of the worker, their competence and well-being in line with Romero's et al. concept of a "healthier" worker. This information is returned to the worker and presented in a readable and understandable manner to identify trends and inform future decisions concerning their work and well-being

Formation of lipofuscin-like autofluorescent granules in the retinal pigment epithelium requires lysosome dysfunction

Funding Information: Supported by Funda??o para a Ciência e Tecnologia (FCT) ? Portugal co-funded by FEDER under the PT2020 Partnership Agreement (to MCS, including project PTDC/MED-PAT/30385/2017, iNOVA4Health-UIDB/04462/2020, research infrastructure PPBI-POCI-01-0145-FEDER-022122, M-ERA.NET 2/0005/2016), Boehringer Ingelheim (to MCS), Fight for Sight UK (to MCS), Wellcome Trust grant number 212216/Z/18/Z/ (to CEF). MJH was funded by Moor-fields Eye Charity with the Bill Brown 1989 Charitable Trust PhD studentship 538158, MLS was funded by FCT-CEECIND/01536/2018, ACF was funded by FCT PhD studentship (PD/BD/135503/2018). This work was developed with the support from the research infrastructure PPBI-POCI-01-0145-FEDER-022122, co-financed by FCT (Portugal) and Lisboa2020, under the PORTUGAL2020 agreement (European Regional Development Fund) and this article is supported by the LYSOCIL project funded by the European Union?s Horizon 2020 programme under grant agreement No. 811087. Funding Information: Supported by Fundação para a Ciência e Tecnologia (FCT) – Portugal co-funded by FEDER under the PT2020 Partnership Agreement (to MCS, including project PTDC/MED-PAT/30385/2017, iNOVA4Health-UIDB/04462/2020, research infrastructure PPBI-POCI-01-0145-FEDER-022122, M-ERA.NET 2/0005/2016), Boehringer Ingelheim (to MCS), Fight for Sight UK (to MCS), Wellcome Trust grant number 212216/Z/18/Z/ (to CEF). MJH was funded by Moor-fields Eye Charity with the Bill Brown 1989 Charitable Trust PhD studentship 538158, MLS was funded by FCT-CEECIND/01536/2018, ACF was funded by FCT PhD studentship (PD/BD/135503/2018). This work was developed with the support from the research infrastructure PPBI-POCI-01-0145-FEDER-022122, co-financed by FCT (Portugal) and Lisboa2020, under the PORTUGAL2020 agreement (European Regional Development Fund) and this article is supported by the LYSOCIL project funded by the European Union’s Horizon 2020 programme under grant agreement No. 811087. Publisher Copyright: Copyright 2021 The AuthorsPURPOSE. We aim to characterize the pathways required for autofluorescent granule (AFG) formation by RPE cells using cultured monolayers. METHODS. We fed RPE monolayers in culture with a single pulse of photoreceptor outer segments (POS). After 24 hours the cells started accumulating AFGs that were comparable to lipofuscin in vivo. Using this model, we used a variety of light and electron microscopical techniques, flow cytometry and Western blot to analyze the formation of AFGs. We also generated a mutant RPE line lacking cathepsin D by gene editing. RESULTS. AFGs seem to derive from incompletely digested POS-containing phagosomes and after 3 days are surrounded by a single membrane positive for lysosome markers. We show by various methods that lysosome-phagosome fusion is required for AFG formation, and that impairment of lysosomal pH or catalytic activity, particularly cathepsin D activity, enhances AF accumulation. CONCLUSIONS. We conclude that lysosomal dysfunction results in incomplete POS degradation and enhanced AFG accumulation.publishersversionpublishe

Proposing a framework to observe and analyze intercultural initiatives

UID/SOC/04647/2013 544410-TEMPUS-1-2013-1-IT-TEMPUS-JPHESIn the present paper we will discuss the contribution of non-formal initiatives to the promotion of an intercultural education, in a holistic perspective of interculturalism and fostering global citizenship. Deepening the analysis of intercultural contexts by taking non-formal initiatives as targets of analysis, it is our objective to propose a framework of observation and evaluation of intercultural initiatives taking place outside the formal educational system. The brief analysis of three intercultural initiatives in two different fields of action – family hosting within an educational context and urban tourism –, taken as examples of non-formal approaches, allowed us to propose a grid analysis as a useful instrument for observation of intercultural relations outside the formal context. This tool can be used within Intercultural Education Platforms on a straight monitoring collaboration with an Observatory of Migrations, complemented by an Observatory of Tolerance that could disseminate the best practices of the considered territory.publishersversionpublishe

reflexões a partir do projecto ALLMEET

UID/SOC/04647/2013; 544410-TEMPUS-1-2013-1-IT-TEMPUS-JPHESNum contexto de diversidade de grupos populacionais em convivência, acelerado pelos processos de globalização das mobilidades, o diálogo intercultural vem-se afirmando como processo educativo de sucesso para o respeito da diversidade e a promoção da coesão social. Considerado uma boa prática da União Europeia (UE), o diálogo intercultural está na base da proposta do projecto ALLMEET, que prevê a implementação de plataformas educativas interculturais em contextos urbanos diferenciados de cinco regiões da Federação Russa. A reflexão e a partilha de experiências no âmbito da rede de trabalho constituída neste projecto conduziu-nos a uma discussão sobre as finalidades educativas e seu enquadramento de grupos minoritários, a criação de territórios educativos prioritários e a interculturalidade em contexto de desigualdades sociais. In a context of sociability of diverse population groups, intercultural dialogue has been affirming as a successful educational idea for the respect of diversity and for the promotion of social cohesion. Considered among the best practices of the European Union, intercultural dialogue is at the basis of the proposal of ALLMEET project, which aims to implement intercultural education platforms in different urban contexts of five regions in Russian Federation. The debate and sharing experiences, within the network constituted in this project, led us to a discussion about educational aims and their framing of minority groups, the creation of priority education territories, and interculturalism in a context of social inequity.publishersversionpublishe

collective monograph

UID/SOC/04647/2013 544410-TEMPUS-1-2013-1-IT-TEMPUS-JPHESpublishersversionpublishe

Characterisation of microbial attack on archaeological bone

As part of an EU funded project to investigate the factors influencing bone preservation in the archaeological record, more than 250 bones from 41 archaeological sites in five countries spanning four climatic regions were studied for diagenetic alteration. Sites were selected to cover a range of environmental conditions and archaeological contexts. Microscopic and physical (mercury intrusion porosimetry) analyses of these bones revealed that the majority (68%) had suffered microbial attack. Furthermore, significant differences were found between animal and human bone in both the state of preservation and the type of microbial attack present. These differences in preservation might result from differences in early taphonomy of the bones. © 2003 Elsevier Science Ltd. All rights reserved

Brazilian Flora 2020: Leveraging the power of a collaborative scientific network

International audienceThe shortage of reliable primary taxonomic data limits the description of biological taxa and the understanding of biodiversity patterns and processes, complicating biogeographical, ecological, and evolutionary studies. This deficit creates a significant taxonomic impediment to biodiversity research and conservation planning. The taxonomic impediment and the biodiversity crisis are widely recognized, highlighting the urgent need for reliable taxonomic data. Over the past decade, numerous countries worldwide have devoted considerable effort to Target 1 of the Global Strategy for Plant Conservation (GSPC), which called for the preparation of a working list of all known plant species by 2010 and an online world Flora by 2020. Brazil is a megadiverse country, home to more of the world's known plant species than any other country. Despite that, Flora Brasiliensis, concluded in 1906, was the last comprehensive treatment of the Brazilian flora. The lack of accurate estimates of the number of species of algae, fungi, and plants occurring in Brazil contributes to the prevailing taxonomic impediment and delays progress towards the GSPC targets. Over the past 12 years, a legion of taxonomists motivated to meet Target 1 of the GSPC, worked together to gather and integrate knowledge on the algal, plant, and fungal diversity of Brazil. Overall, a team of about 980 taxonomists joined efforts in a highly collaborative project that used cybertaxonomy to prepare an updated Flora of Brazil, showing the power of scientific collaboration to reach ambitious goals. This paper presents an overview of the Brazilian Flora 2020 and provides taxonomic and spatial updates on the algae, fungi, and plants found in one of the world's most biodiverse countries. We further identify collection gaps and summarize future goals that extend beyond 2020. Our results show that Brazil is home to 46,975 native species of algae, fungi, and plants, of which 19,669 are endemic to the country. The data compiled to date suggests that the Atlantic Rainforest might be the most diverse Brazilian domain for all plant groups except gymnosperms, which are most diverse in the Amazon. However, scientific knowledge of Brazilian diversity is still unequally distributed, with the Atlantic Rainforest and the Cerrado being the most intensively sampled and studied biomes in the country. In times of “scientific reductionism”, with botanical and mycological sciences suffering pervasive depreciation in recent decades, the first online Flora of Brazil 2020 significantly enhanced the quality and quantity of taxonomic data available for algae, fungi, and plants from Brazil. This project also made all the information freely available online, providing a firm foundation for future research and for the management, conservation, and sustainable use of the Brazilian funga and flora